By Christine Gagliardi, Bruce A. Bunnell (auth.), Jeffrey M. Gimble, Bruce A. Bunnell (eds.)
During the earlier decade, a variety of clinical disciplines have followed using adipose-derived stem/stromal cells (ASCs) as a huge device for examine and discovery. In Adipose-Derived Stem Cells: equipment and Protocols, specialists from the sphere, together with individuals of the esteemed overseas Federation of Adipose Therapeutics and technology (IFATS), offer outlined and proven protocols on the way to extra codify the usage of those strong and available cells. With chapters geared up round techniques spanning the invention, pre-clinical, and scientific procedures, a lot of the emphasis is put on human ASC, whereas extra strategies concerning small and big animal species are incorporated. As a quantity within the hugely profitable tools in Molecular Biology™ sequence, the distinctive contributions comprise introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, with ease reproducible laboratory protocols, and notes on troubleshooting and warding off recognized pitfalls. complete and state of the art, Adipose-Derived Stem Cells: tools and Protocols serves as a necessary reference textual content for knowledgeable researchers in addition to new scholars at the route to extra exploring the wonderful strength of ASCs.
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Additional resources for Adipose-Derived Stem Cells: Methods and Protocols
Therefore a filtration procedure can be performed by using a nylon mesh filter with a small pore size (25 mm). References 1. Rangwala, S. M. and Lazar, M. A. (2000) Transcriptional control of adipogenesis. Annu Rev Nutr 20, 535–59. 2. , and Ailhaud, G. (1987) Differentiation of human adipocyte precursors in a chemically defined serum-free medium. Int J Obes 11, 19–27. 3. , and Pfeiffer, E. F. (1989) Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells cultured in a chemically defined medium.
2-mm syringe filter. Store at room temperature covered with aluminum foil to protect from the light. 7. , Minneapolis, MN): Add antibiotic solution to StemXVivo chondrogenic base media at a 1:100 dilution (100 mL antibiotic + 9,900 mL base media). Store at 4°C in the dark. ): Add StemXVivo Chondrogenic Supplement to the complete chondrogenic base media at a 1:100 dilution (100 mL supplement + 9,900 mL completed base media). Store at 4°C in the dark. In Vitro Adult Rat Adipose Tissue-Derived Stromal Cell Isolation and Differentiation 41 3.
Putnam, L. , Popescu, I. , and Bunnell, B. A. (2004) Neurogenesis of rhesus adipose stromal cells. J Cell Sci 117, 4289–99. , Gimble, J. , and Bunnell, B. A. (2006) Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue. J Cell Biochem 99, 1285–97. 12. Rodbell, M. (1966) Metabolism of isolated fat cells. The similar effects of phospholipase C (Clostridium perfringens alpha toxin) and of insulin on glucose and amino acid metabolism. J Biol Chem 241, 130–9. 13.
Adipose-Derived Stem Cells: Methods and Protocols by Christine Gagliardi, Bruce A. Bunnell (auth.), Jeffrey M. Gimble, Bruce A. Bunnell (eds.)
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