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When the cycle is complete, remove the glass tube outlet from the test tube and stir the hydroxide of Hyamine solution. Seal the tube and keep for the determination of total 14C activity. Prepare a blank by repeating the entire procedure using non-labeled plant tissue. Sample analysis Reagents 1) Toluene ethanol scintillator. , Ltd. see above). 2) 14 C-sodium bicarbonate standards (for the determination of counting efficiency). 05 ┬Ác/ml in 'alkaline' distilled water. Prepare the standards (below) by placing the amount of NaH 14 CO3 stock solution indicated in a glass vial of the type used in the liquid scintillation spectrometer.

5 N KOH to the separating funnel and allow to run into the beaker. Leave for 10 minutes to absorb most of the excess 14CO2 . Then remove the wooden-mylar frame. Harvest the plants according to the requirements of the experiment. Comments The total activity of the NaH 14CO3 solution to be used will depend on the purpose of the experiment. Adding 200 uc per chamber 10 days before flowering gives adequate activity for measuring starch, sugar and total 14C activity until grain maturity. Be Sure the NaH 14 CO3 is made up in alkaline distilled water to prevent loss of 14CO 2 .

Sci. Section B, 49:386-396. Tanaka, A. and S. Yoshida. 1970. Nutritional disorders of rice in Asia. Int. Rice Res. Inst. Tech. Bull. 10. 51 p.

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Aircrew Manual - Fairey Swordfish MK II [AP 1517B-PN]


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