By Guozhong Cao, Qifeng Zhang, C. Jeffrey Brinker

ISBN-10: 9814280518

ISBN-13: 9789814280518

Annual assessment of Nano study, quantity three focuses customarily on nanofabrication, nanomaterials and nanostructures, and effort program of nanomaterials. the entire evaluate chapters are contributed by way of well-published scientists and convey the latest development in chosen themes to the readers. This assessment quantity will serve twin reasons: both as a very good advent to scientists whose services lie in numerous fields yet who're drawn to studying approximately nanotechnology, or as a short reference for specialists energetic within the box of nanoscience and nanotechnology.

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36 Leifert et al. 3. Nanoimprint Lithography Nanoimprint lithography [248-250] and closely related techniques [251-253] facilitate imprinting of a rigid nanostructured mold into a thin layer of spin-coated resist. The excess resist is then removed in the deformed areas via reactive ion etching (pattern transfer). There are different ways that nanostructures created via imprint lithography can be used for biosensing platforms. They have been utilized in a “lab on a chip” device providing a nanoscaled channel network.

Plasmonic biosensors or gold nanoparticles (20 nm) have been functionalized with an anti-actin antibody and a TAT-HA2 peptide, which mediated the endocytotic uptake of the nanoparticles into the cell, and their subsequent release from endosomes into the cytoplasm [84]. Binding of the nanosensors to actin could be measured since this brought the probes into such close proximity that the plasmon resonance became red-shifted, which could be detected by darkfield reflectance imaging or confocal microscopy, with detection limited to between 623-643 nm.

Using this approach, nanoarrays of DNA [295,296], peptides [297,298], proteins [299,300], lipids [301], viruses [302-304] and bacteria [305] have been created. In addition, enzymes patterned via DPN have been used to perform localized reactions on a surface [306308]. In order to produce nanoarrays for parallel high-throughput screening, the inherent slowness of the process when using a single AFM tip is being overcome by either using an array of individually actuated tips [309-311] or of passive tips.

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Annual Review of Nano Research, Volume 3 by Guozhong Cao, Qifeng Zhang, C. Jeffrey Brinker


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